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Stem cells to treat liver failurePosted on 8/6/2008 at 7:37 AM - Post CommentTransplantation of liver cells - a promising method of treatment of acute and chronic liver failure.Japanese scientists from Okayama Gakuin University investigated the possible use for the treatment of liver failure gepatotsitopodobnyh cells derived from bone marrow stem cells. For this rat stem cells from bone marrow was obtained a stable cell line bystrodelyaschihsya of cells. At least over 300 doubling the number (about 300 days) cells maintained normal diploid karyotype and not changed their basic properties. The cells express markers, indicating their origin mezenhimalnom: CD29, CD44, CD49b, CD90, vimentin and fibronectin, but not CD45. When growing at Matrigele (Matrigel) with the addition of hepatocyte growth factor and fibroblast growth factor-4, cells differentiated into gepatotsitopodobnye cells, expressing albumin, cytochrome-P450 (CYP) 1A1, CYP1A2, glucose-6-phosphatase, tryptophan-2, 3 -- dioksigenazu, tirozinaminotransferazu, nuclear factors hepatocytes 1 - alpha and 4 - Alpha. Transplantation of the spleen cells in rats that have been removed 90% of the liver, prevents the development of hepatic failure (without treatment such rats are killed). The results indicate the possibility of using bone marrow stem cells for the treatment of severe liver diseases. Materials research presented in the article Miyazaki M, et al. Isolation of a Bone Marrow-Derived Stem Cell Line with High Proliferation Potential and Its Application for Preventing Acute Fatal Liver Failure. Stem Cells. 2007 Aug 16; |
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